Printed Digital Wax-up Model as a Blueprint for Layered Pressed-ceramic Laminate Veneers: Technique Description and Case Report.

This article presents a clinical technique for transferring Digital Smile Design (DSD) information and a digital wax-up to fabricate highly personalized ceramic laminate veneers. The hybrid workflow of digitally designed wax-up and conventional hand-crafted veneers is showcased in a female adult who sought to improve her smile due to maxillary anterior incisal wear. The ceramic veneers, layered by feldspathic porcelain, followed the contours and shade blueprint planned with the digital wax-up. The DSD improved the harmony of the facial-and-smile relationship, and the ceramic restorations fulfilled the patient's esthetic and functional demands. Guides based on the printed model with the digitally designed wax-up can provide a reference for pressed lithium disilicate veneers layered with feldspathic porcelain.

Injectable Flowable Resin-based Composite Veneers Prior to Ceramic Veneers.

OBJECTIVES: This case report describes the clinical protocols for using injectable flowable resin-based composite veneers without tooth reduction as long-term prototype restorations, followed by conservative feldspathic veneer restorations. CLINICAL CONSIDERATION: The patient's primary concern was to improve her smile. After clinical evaluation, injectable flowable resin composite veneers were recommended as long-term prototypes followed by conservative tooth preparation and ceramic veneers. Flowable composite veneers were created with a transparent silicone index fabricated from a diagnostic wax-up approved by the patient. No tooth reduction was required to place this type of composite veneer. Later, a printed reduction guide was used to provide conservative tooth preparation followed by the cementation of thin feldspathic porcelain veneers under full isolation with a rubber dam. CONCLUSIONS: Injectable flowable resin-based composite veneers can successfully act as long-term esthetic prototypes before ceramic veneers. The outcome of these restorations is very predictable because the diagnostic wax-up anatomy is transferred to the mouth through the transparent silicone index, and they can be placed without tooth reduction. Conservative tooth preparation can be performed on the composite veneers so that minimal tooth structure is removed to maximize the bonding performance of ceramic veneers.

Ultrathin Partial Ceramic Veneers in the Esthetic Zone with Tooth Whitening: A 5-year Case Report.

OBJECTIVE: This case report describes a minimally invasive approach with tooth whitening and partial ceramic veneers in the esthetic zone with a 5-year follow-up. CLINICAL CONSIDERATIONS: The patient was initially concerned about the tooth color and previous direct resin composite restorations that chipped on the incisal edges of both maxillary central incisors. Tooth whitening and partial veneers for both central incisors were recommended after clinical evaluation. Two sessions of in-office tooth whitening, first with 35% hydrogen peroxide and later with 10% carbamide peroxide from first premolar to first premolar, were provided. Minimal tooth preparation that only removed the fractured composite restorations was performed followed by placement of feldspathic porcelain ultrathin partial ceramic veneers for both central incisors. We emphasize the benefits of minimal tooth preparation paired with partial ceramic veneers and the importance of masking discolored tooth structure with such thin veneers, including potential tooth whitening. CONCLUSIONS: Overall, we demonstrate a well-planned and executed restorative procedure combining tooth whitening and ultrathin partial ceramic veneers to achieve desired results in the esthetic zone that remained successful for five years.

Polychromatic Composite and Resin Infiltration Restorations in the Esthetic Zone: A Five-year Clinical Report.

OBJECTIVE: This case report describes the use of a combination of a polychromatic resin composite restoration and resin infiltration to achieve a satisfactory esthetic outcome that has lasted over five years. CLINICAL CONSIDERATIONS: The patient's primary complaint was a desire to replace an existing stained resin composite on the facial and mesial surfaces of the maxillary left central incisor, and a white spot on the facial surface of the maxillary right central incisor. After clinical evaluation, the patient was offered either replacement of the restoration with a polychromatic restoration simulating a white spot, or a combined treatment with polychromatic composite and resin infiltration restorations, which was recommended. The patient selected the latter treatment. A putty matrix was fabricated, and a quick mock-up was done using various enamel and dentin shades of resin composite to determine proper material selection. The previous resin composite restoration was removed, and layers of new dentin, body, and enamel composite were placed. An interproximal matrix was used in order to provide ideal contour and contact. Finishing and polishing procedures were performed. The maxillary right central incisor was treated with resin infiltration in order to subdue the white spot on the facial surface. CONCLUSIONS: Polychromatic resin composite and resin infiltration restorations to replace a stained restoration and mask a white spot can achieve desired results in the esthetic zone and maintain them for five years.

Bloqueo costoclavicular del plexo braquial: una revisión de la evidencia actual / Costoclavicular brachial plexus block: A review of current evidence

El bloqueo del plexo braquial puede realizarse mediante varios abordajes. El bloqueo costoclavicular ecoguiado en el espacio costoclavicular (CCS) descrito recientemente ha ganado popularidad, presentando resultados prometedores para la anestesia del miembro superior y la analgesia postoperatoria. Este bloqueo se presenta como una alternativa al abordaje infraclavicular tradicional, con resultados que muestran una mejor calidad del bloqueo y un menor riesgo de complicaciones. Este artículo presenta una revisión narrativa de la literatura y la evidencia actual disponible. Se realizó una búsqueda bibliográfica en la base de datos PubMed abarcando los artículos relevantes publicados entre 2015 y diciembre de 2020.(AU)

The brachial plexus block can be performed through several approaches. The recently described ultrasound-guided costoclavicular block at the costoclavicular space has gained popularity, presenting promising results for upper limb anesthesia and postoperative analgesia. This block comes as an alternative to the traditional infraclavicular approach, with results suggesting improved block quality and reduced risk of complications. This article presents a narrative review of the literature and the current evidence available. A bibliographic search was conducted in the database PubMed and relevant articles published between 2015 and December 2020 to this topic were selected.(AU)

Costoclavicular brachial plexus block: A review of current evidence.

The brachial plexus block can be performed through several approaches. The recently described ultrasound-guided costoclavicular block at the costoclavicular space has gained popularity, presenting promising results for upper limb anaesthesia and postoperative analgesia. This block comes as an alternative to the traditional infraclavicular approach, with results suggesting improved block quality and reduced risk of complications. This article presents a narrative review of the literature and the current evidence available. A bibliographic search was conducted in the database PubMed and relevant articles published between 2015 and December 2020 to this topic were selected.

Minimally Invasive Multidisciplinary Restorative Approach to the Esthetic Zone Including a Single Discolored Tooth.

OBJECTIVES: The case report describes a minimally invasive, multidisciplinary approach to a single discolored anterior tooth, with internal bleaching using traditional Japanese paper (Washi), a gingivoplasty with a three-dimensional (3D) printed surgical guide, and ultrathin feldspathic porcelain veneers. CLINICAL CONSIDERATION: The patient's primary concern was improving her smile. After clinical evaluation, internal tooth bleaching for the discolored tooth and gingivoplasty with restoration of the maxillary anterior six teeth and first premolars was recommended. The internal tooth whitening was accomplished with sodium perborate mixed with 30% hydrogen peroxide impregnated in Washi and sealed in the root canal with glass ionomer. Once the tooth bleaching was completed, the 3D printed surgical guide was placed in the patient's maxillary anterior region and used to guide soft tissue recontouring. After 6 months, ultrathin feldspathic porcelain veneers were placed. CONCLUSION: Well-planned restorative procedures combining internal tooth bleaching using Washi, gingivoplasty performed with electrosurgery using a 3D printed surgical guide, and ultrathin feldspathic porcelain veneers can achieve the desired results in the esthetic zone and remain successful for 4 years.

A Conservative Approach to Ceramic Veneers: A Case Report.

Bonding to enamel has been shown to provide reliable results, and thus conservative tooth preparations are key to the success of ceramic bonded restorations. The wax-up is the first diagnostic tool available to evaluate discrepancies between current and ideal tooth proportions. The clinician's diagnostic mock-up provides the patient with a visual perception of the size and shape of the proposed restorations. The use of reduction guides assists the restorative dentist in evaluating the specific amount of tooth structure to be removed during preparation. Furthermore, total isolation with a rubber dam prior to bonding the final restorations is crucial for the success of adhesive protocols. The aim of this report is to demonstrate a conservative approach to tooth preparation with a complete isolation technique prior to bonding eight ceramic restorations.

A role for MALT1 activity in Kaposi's sarcoma-associated herpes virus latency and growth of primary effusion lymphoma.

Primary effusion lymphoma (PEL) is an incurable malignancy that develops in immunodeficient patients as a consequence of latent infection of B-cells with Kaposi's sarcoma-associated herpes virus (KSHV). Malignant growth of KSHV-infected B cells requires the activity of the transcription factor nuclear factor (NF)-κB, which controls maintenance of viral latency and suppression of the viral lytic program. Here we show that the KSHV proteins K13 and K15 promote NF-κB activation via the protease mucosa-associated lymphoid tissue lymphoma translocation protein-1 (MALT1), a key driver of NF-κB activation in lymphocytes. Inhibition of the MALT1 protease activity induced a switch from the latent to the lytic stage of viral infection, and led to reduced growth and survival of PEL cell lines in vitro and in a xenograft model. These results demonstrate a key role for the proteolytic activity of MALT1 in PEL, and provide a rationale for the pharmacological targeting of MALT1 in PEL therapy.

Retinoblastoma-binding protein 2 (RBP2) is frequently expressed in neuroendocrine tumors and promotes the neoplastic phenotype.

Neuroendocrine tumors (NETs), which can have survival rates as low as 4%, currently have limited therapeutic interventions available highlighting the dire need for the identification of novel biological targets for use as new potential drug targets. One such potential target is retinoblastoma-binding protein 2 (RBP2), an H3K4 demethylase whose overexpression has been linked to cancer formation and metastasis in non-endocrine tumor types. We measured RBP2 mRNA and protein levels in enteropancreatic NETs by measuring RBP2 in matched human normal and NET tissue samples. Further, proliferation, migration, invasion and colony formation assays were performed in the physiologically relevant NET cell lines ßlox5, H727 and QGP-1 to understand the role of RBP2 and its demethylase activity on end points of tumorigenesis. Our data indicate a strong correlation between RBP2 mRNA and protein expression in NET specimens. RBP2 was overexpressed relative to tissue-matched normal controls in 80% of the human tumors measured. In vitro studies showed RBP2 overexpression significantly increased proliferation, migration, invasion and colony formation, whereas knockdown significantly decreases the same parameters in a demethylase-independent manner. The cell cycle inhibitors p21 and p57 decreased with RBP2 overexpression and increased upon its depletion, suggesting a regulatory role for RBP2 in cellular proliferation. Taken together, our results support the hypothesis that the aberrant overexpression of RBP2 is a frequent contributing factor to tumor formation and metastasis in enteropancreatic NETs.

Prevalencia del dolor no oncológico en la consulta de rehabilitación / The prevalence of non-cancer pain in rehabilitation practice

Introducción. El dolor es el síntoma acompañante que provoca con mayor frecuencia una consulta médica. El dolor crónico es un tema de gran relevancia para los profesionales de la salud, el paciente y la sociedad. Objetivo. Valorar la prevalencia de dolor en las consultas del médico rehabilitador. Material y métodos. Estudio descriptivo prospectivo, observacional multicéntrico en 3 hospitales. Se valoraron pacientes con dolor entre el 1.1.2012 y el 28.2.2012. Se analizaron: variables sociodemográficas, antropométricas y características del dolor. Resultados. El estudio incluyó a 1.168 pacientes: 974 padecían algún tipo de dolor, de los cuales el 62,5% son mujeres; la mayoría de pacientes padecía dolor en las extremidades superiores (32,1%) y la mitad de los pacientes presentaron dolor crónico, siendo los >58 años los que con más frecuencia sufrían dolor crónico (p > 0,001). La intensidad del dolor en la escala VAS fue de 54,70 y de 6,16 en la NRS. El 70,7% recibían tratamiento analgésico. Un 64,1% no realizaba ninguna actividad física. Conclusiones. Los resultados de nuestro estudio demuestran una alta prevalencia de dolor en las consultas del médico rehabilitador. Se podría considerar nuestra especialidad como referente en el manejo y tratamiento del dolor (AU)

Introduction. Pain is the most common symptom prompting medical consultation. Chronic pain is a highly important issue for health professionals, patients and society. Objective. To assess the prevalence of pain in rehabilitation practice. Material and methods. This descriptive, prospective, multicenter study evaluated pain in patients in 3 hospitals between 1st January, 2012 and 28th February, 2012. We analyzed sociodemographic and anthropometric variables and pain features. Results. The study included 1168 patients; 974 had some kind of pain, of which 62.5% were women. Most patients had pain in the upper extremities (32.1%). Half the patients had chronic pain. Pain was most common in patients aged >58 years (P<.001). Pain intensity on the VAS scale was 54.70 mm and 6.18 on the NRS. A total of 70.7% were receiving analgesics and 64.1% performed no physical activity. Conclusions. The results of our study demonstrate a high prevalence of pain in rehabilitation consultation. Our speciality could be considered as pivotal in the management and treatment of pain (AU)

Puesta en marcha de una Unidad de Ortogeriatría en un hospital de segundo nivel. Resultados clínicos y funcionales a corto y medio plazo / Start-up of an Orthogeriatric Unit in a general hospital. Short and medium-term clinical and functional outcomes

Introducción. En el año 2010 creamos la Unidad de Ortogeriatría (UOG) de nuestro hospital, una iniciativa multidisciplinaria que ofrece una asistencia continuada y especializada para ancianos con fractura de cadera (FC). Objetivo. Describir el funcionamiento de la unidad y evaluar las principales características médicas y funcionales de los pacientes ingresados. Material y método. Estudio descriptivo retrospectivo que incluye a los pacientes con FC osteoporótica ingresados desde noviembre del 2010 hasta abril del 2012. Se recogen variables sociodemográficas, médicas y funcionales durante el ingreso, al alta y a los 3 meses del alta. Resultados. Ciento noventa pacientes, con una media de edad 84 años, 74,2% mujeres, 91,1% procedentes de domicilio, 74,7% autónomos o levemente dependientes para las actividades básicas de la vida diaria (ABVD) y el 80,1% realizaba marcha independiente o supervisada. Estancia media prequirúrgica 5,4 días y total 14,7, por encima de las de los estudios de otras UOG pero inferiores a las de los pacientes atendidos por FC en nuestro hospital durante el 2009. Mortalidad intrahospitalaria 1,1%, inferior a la de los pacientes ingresados por FC en nuestro hospital durante el 2009. A los 3 meses eran autónomos o dependientes leves para las ABVD, el 48,3% de los pacientes y el 60,2% realizaba marcha independiente o supervisada. Conclusión. La UOG parece aportar mejoras en la evolución clínica y funcional del paciente anciano con FC y una disminución de la estancia hospitalaria, reduciéndose así los costes asistenciales. Estos resultados invitan a seguir con este sistema de atención del anciano con FC (AU)

Introduction. An Orthogeriatric Unit (UOG) was created in our hospital in 2010. This is a multidisciplinary initiative that provides continuous specialized care for elderly persons with hip fracture (HF). Objective. To describe the operation of the unit and evaluate the medical and functional characteristics of admitted patients. Material and method. A retrospective descriptive study was conducted of all patients with osteoporotic HF admitted from November 2010 to April 2012. We collected socio-demographic, medical and functional variables during admission, at discharge, and 3 months after discharge. Results. A total of 190 patients were included, with a mean age of 84 years. Of these, 74.2% were women, 91.1% were admitted from home, 74.7% were independent or slightly dependent for basic activities of daily living (BADL), and 80.1% performed independent or supervised ambulation. Mean pre-surgical length of stay was 5.4 days and the total length of stay was 14.7 days, which was higher than rates reported in other UOG studies but lower than our hospital data for 2009. In-hospital mortality was 1.1%, representing a decrease compared with patients admitted to our hospital for HF in 2009. At 3 months after discharge, 48.3% of the patients were independent or slightly dependent for BADL and 60.2% were able to walk independently or with supervision. Conclusion. UOG appears to improve clinical and functional outcomes in elderly persons with HF and to decrease length of hospital stay, thus reducing healthcare costs. These results support the continued use of this care system for elderly persons with HF (AU)

Stillbirth history and Toxoplasma gondii infection in women attending public health centers in a northern Mexican City.

Through a cross-sectional study design, 150 women attending public health centers with a history of stillbirths were examined for anti-Toxoplasma gondii IgG and IgM antibodies in Durango City, Mexico. Bivariate and multivariate analyses were used to assess the association of T. gondii seropositivity with the characteristics of the women with stillbirth history. Of the 150 women (mean age: 32.09 ± 9.16 years) studied, 14 (9.3%) had anti-T. gondii IgG antibodies and six (42.9%) of them were also positive for anti-T. gondii IgM antibodies. Multivariate analysis showed that T. gondii seropositivity was associated with high frequency (4-7 days a week) of eating meat (OR = 5.52; 95% CI: 1.48-20.59; P = 0.01), history of lymphadenopathy (OR = 4.52; 95% CI: 1.14-17.82; P = 0.03), and history of surgery (OR = 8.68; 95% CI: 1.04-72.15; P = 0.04). This is the first study on the seroepidemiology of T. gondii infection in women with a history of stillbirths in Mexico. The association of T. gondii exposure with a history of surgery warrants for further research. Risk factors for T. gondii infection found in the present survey may help to design optimal educational programs to avoid T. gondii infection.

Miscarriage history and Toxoplasma gondii infection: A cross-sectional study in women in Durango City, Mexico.

Through a cross-sectional study design, 326 women with a history of miscarriage were examined for anti-Toxoplasma gondii IgG and IgM antibodies in Durango City, Mexico. Prevalence association with sociodemographic, clinical, and behavioral characteristics in women with miscarriage was also investigated. Twenty-two (6.7%) of the 326 women studied had anti-T. gondii IgG antibodies and two (0.6%) were also positive for anti-T. gondii IgM antibodies. Seroprevalence of T. gondii infection was not influenced by age, birth place, occupation, educational level, or socioeconomic status. In contrast, logistic regression showed that T. gondii exposure was associated with consumption of raw or undercooked meat (OR = 6.84; 95% CI: 1.04-44.95; P = 0.04) and consumption of chicken brains (OR = 18.48; 95% CI: 1.26-269.43; P = 0.03). This is the first study on the seroepidemiology of T. gondii infection in women with a history of miscarriage in Northern Mexico. Of interest, we also observed an association of T. gondii exposure with consumption of chicken brains. Contributing factors for T. gondii exposure found in the present study should be taken into consideration for public health measures to avoid infection with T. gondii and its sequelae.

High seroprevalence of Toxoplasma gondii infection in inmates: A case control study in Durango City, Mexico.

PURPOSE: The seroprevalence of infection with the parasite Toxoplasma gondii and the association with risk factors has not been determined in inmates. Through a case-control study, 166 inmates from a state correctional facility in Durango City, Mexico and 166 age- and gender-matched non-incarcerated subjects were examined for the presence of anti-T. gondii IgG and IgM antibodies using enzyme-linked immunoassays. RESULTS: Seroprevalence of anti-T. gondii IgG antibodies was higher in inmates (35, 21.1%) than in controls (14, 8.4%) (OR = 2.90; 95% CI: 1.43-5.94; P = 0.001). Anti-T. gondii IgM antibodies were detected in two (1.2%) inmates and in seven (4.2%) controls (P = 0.17). Multivariate analysis of socio-demographic, incarceration, and behavioral characteristics of inmates revealed that T. gondii seropositivity was associated with being born out of Durango State (OR = 3.91; 95% CI: 1.29-11.79; P = 0.01). In addition, T. gondii seroprevalence was higher (P = 0.03) in inmates that had suffered from injuries (17/56: 30.4%) than those without such history (18/110: 16.4%). CONCLUSIONS: The seroprevalence of T. gondii infection in inmates in Durango City is higher than the seroprevalences found in the general population in the same city, indicating that inmates may represent a new risk group for T. gondii infection. Further research on T. gondii infection in inmates is needed.

Accidental spinal potassium chloride injection successfully treated with spinal lavage.

We describe the management of a 62-year-old man who developed severe pain, cramps, paraplegia and pulmonary oedema after the accidental administration of potassium chloride into the subarachnoid space. In addition to supportive treatment, we performed cerebrospinal fluid lavage with saline 0.9%. The patient recovered well without any permanent injury.

Apical extrusion of bacteria when using reciprocating single-file and rotary multifile instrumentation systems.

AIM: To evaluate ex vivo, apical bacterial extrusion associated with two reciprocating single-file systems (WaveOne and Reciproc) compared with a conventional multifile rotary system (BioRace). METHODOLOGY: Forty-five human single-rooted mandibular incisors were used. Endodontic access cavities were prepared, and root canals were contaminated with an Enterococcus faecalis suspension. Following incubation at 37 °C for thirty days, the contaminated teeth were divided into three groups of 15 specimens each (G1 - Reciproc, G2 - WaveOne and G3 - BioRace). Positive and negative controls consisted of 5 infected teeth and 3 uninfected incisors that were instrumented with one of the tested NiTi systems, respectively. Bacteria extruded from the apical foramen during instrumentation were collected into vials containing 0.9% NaCl. The microbiological samples were taken from the vials and incubated in brain heart agar medium for 24 h. The resulting bacterial titre, in colony-forming units (CFU) per mL, was determined, and these data were analysed by Wilcoxon matched-pairs signed rank test and Kruskal-Wallis H-test. The level of significance was set at α = 0.05. RESULTS: No significant difference was found in the number of CFU between the two reciprocating systems (P = 0.41). The conventional multifile rotary system group was associated with significantly higher CFU than both of the two reciprocating groups (P = 0.01). CONCLUSIONS: All instrumentation systems extruded bacteria beyond the foramen. However, both reciprocating single-file systems extruded fewer bacteria apically than the conventional multifile rotary system.

Donor characteristics that are associated with survival in liver transplant recipients older than 70 years with grafts.

INTRODUCTION: The use of grafts from donors older than 70 years of age is increasing due to the decrease in the number of donors and the increase in waiting list patients. MATERIAL AND METHODS: We undertook a univariate and multivariate analysis of 980 adult recipients of whole liver grafts, 129 of them from donors aged 70 years or older. RESULTS: No differences were found in patient survival compared with recipients of younger grafts. There were no higher rates of rejection, vascular or biliary complications, postoperative bleeding, or infections, but older grafts were associated with graft dysfunction (P = .01) and a higher frequency of postoperative refractory ascites (P = .007), but without a greater need for retransplantation. As graft-associated factors, the joint presence in the donor of diabetes (P = .00; confidence interval [CI] = 0.04-0.117), hypertension (P = .00; CI = 0.22-0.39), and weight of more than 90 kg (P = .031; CI = 0.05-0.104) were suggestive of poor prognostic factors in recipient survival. Survival in hepatitis C virus (HCV) recipients or recipients aged older than 60 years was worse with donors aged older than 70 years, although not significantly so. With grafts from donors aged older than 80 years (n = 15), although patient survival rate was good (70% at 10 years), there was a higher rate of retransplantation (20%) and the early mortality rate was 13.3%. CONCLUSIONS: Use of grafts from donors aged older than 70 years is safe, with similar survival to patients with younger grafts. The appearance of initial dysfunction with prolonged ascites may be due to a delay in reaching a correct functionality, but was not associated with increased mortality, complications, or need for retransplantation. It should also be avoided in recipients older than 60 years or with HCV. Grafts older than 80 years were associated with a good long-term patient survival but at the expense of a higher rate of retransplantation. However, it helps to reduce the time on the waiting list and, thus, mortality. We noted decreased survival associated with donor hypertension, diabetes, and obesity, so these donors should be selected more rigorously.

Outbreak of nosocomial sepsis and pneumonia in a newborn intensive care unit by multiresistant extended-spectrum beta-lactamase-producing Klebsiella pneumoniae: high impact on mortality.

We describe a case-control study of a small outbreak of nosocomial sepsis and pneumonia with high mortality due to clonal dissemination of a multiresistant Klebsiella pneumoniae in the neonatal intensive care unit of a Mexican institution. Our study helped to change nosocomial infection control policy in this hospital.